A peptide inhibitor of MurA UDP-N-acetylglucosamine enolpyruvyl transferase: the first committed step in peptidoglycan biosynthesis.
Identifieur interne : 002D55 ( Main/Exploration ); précédent : 002D54; suivant : 002D56A peptide inhibitor of MurA UDP-N-acetylglucosamine enolpyruvyl transferase: the first committed step in peptidoglycan biosynthesis.
Auteurs : José Molina-L Pez [Mexique] ; François Sanschagrin ; Roger C. LevesqueSource :
- Peptides [ 0196-9781 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Alkyl et aryl transferases.
- biosynthèse : Peptidoglycane.
- enzymologie : Pseudomonas aeruginosa.
- métabolisme : Alkyl et aryl transferases, Uridine diphosphate ose.
- physiologie : Peptides.
- Données de séquences moléculaires, Séquence d'acides aminés.
English descriptors
- KwdEn :
- MESH :
- chemical , antagonists & inhibitors : Alkyl and Aryl Transferases.
- chemical , biosynthesis : Peptidoglycan.
- chemical , metabolism : Alkyl and Aryl Transferases, Uridine Diphosphate Sugars.
- chemical , physiology : Peptides.
- enzymology : Pseudomonas aeruginosa.
- Amino Acid Sequence, Molecular Sequence Data.
Abstract
The MurA enzyme from Pseudomonas aeruginosa was purified to homogeneity and found to be biologically active as a UDP-N-acetylglucosamine (UNAG) enolpyruvyl transferase in a coupled enzyme assay where ATPase activity was measured by the release of inorganic phosphate. A microtiter plate assay coupled to competitive biopanning using the UDP-N-acetylglucosamine was used to screen 10(9) C-7-C and 12-mers peptides from phage display libraries. From 60 phage-encoded peptides identified after the fourth round of biopanning, deduced amino acid sequences were aligned and two peptides were synthesized and tested for inhibition of the MurA-catalyzed reaction. The PEP 1354 peptide inhibited the ATPase activity of MurA with an IC(50) value of 200muM and was found to be a competitive inhibitor of UNAG. The pre-incubation of MurA with inhibitor indicated a time-independent inhibition. This time-dependent inhibition is the first report of peptide inhibitors of MurA, which represent the scaffold for the synthesis of inhibitory peptidomimetic molecules.
DOI: 10.1016/j.peptides.2006.08.023
PubMed: 17030076
Affiliations:
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Le document en format XML
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<term>Molecular Sequence Data</term>
<term>Peptides (physiology)</term>
<term>Peptidoglycan (biosynthesis)</term>
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<term>Peptides (physiologie)</term>
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<term>Séquence d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">The MurA enzyme from Pseudomonas aeruginosa was purified to homogeneity and found to be biologically active as a UDP-N-acetylglucosamine (UNAG) enolpyruvyl transferase in a coupled enzyme assay where ATPase activity was measured by the release of inorganic phosphate. A microtiter plate assay coupled to competitive biopanning using the UDP-N-acetylglucosamine was used to screen 10(9) C-7-C and 12-mers peptides from phage display libraries. From 60 phage-encoded peptides identified after the fourth round of biopanning, deduced amino acid sequences were aligned and two peptides were synthesized and tested for inhibition of the MurA-catalyzed reaction. The PEP 1354 peptide inhibited the ATPase activity of MurA with an IC(50) value of 200muM and was found to be a competitive inhibitor of UNAG. The pre-incubation of MurA with inhibitor indicated a time-independent inhibition. This time-dependent inhibition is the first report of peptide inhibitors of MurA, which represent the scaffold for the synthesis of inhibitory peptidomimetic molecules.</div>
</front>
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<country name="Mexique"><noRegion><name sortKey="Molina L Pez, Jose" sort="Molina L Pez, Jose" uniqKey="Molina L Pez J" first="José" last="Molina-L Pez">José Molina-L Pez</name>
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